Testing for evidence of philopatry: minisatellite DNA variation in Auckland Island teals
نویسندگان
چکیده
This study examined minisatellite DNA variation in three groups of Auckland Island teals from three locations (Boat Bay, Nellie Spit and East Coast). To determine whether teals showed high levels of natal philopatry, we were asked to test two hypotheses: 1) birds within each group are more closely related to each than to those in other groups; and 2) birds from nearby sites, Boat Bay and Nellie Spit, are more closely related to each other than to those from the more distant East Coast group. DNA was extracted from whole blood and the samples were digested with the restriction enzyme HaeIII . The digested DNA was probed with three multilocus minisatellite DNA probes, namely 33.15, pV47-2 and 3'HVR. DNA profiles revealed high levels of bandsharing between individuals within each of the three groups of Auckland Island teal studied (range 0.645 0.765). Interestingly, bandsharing levels between individuals from different groups were very similar to those recorded within groups (range 0.633 0.739). Thus, there is no evidence to indicate that the Boat Bay and Nellie Spit samples are more closely related to each other than they are to birds from the East Coast group. One family of Auckland Island teals allowed bandsharing indices to be calculated for individuals of known relationships. The parent offspring levels of bandsharing were generally higher than those obtained for within and between Ewing Island groups.The results presented here suggest that any philopatry in Auckland Island teals has not resulted in detectable genetic differences between groups, as revealed by minisatellite DNA markers.
منابع مشابه
DNA Fingerprinting Studies of Teals and Ducks A report prepared for the Department of Conservation
The multilocus DNA fingerprinting technique has been applied to a range of tissue samples from sub-antarctic teals and hyrids between Grey and Mallard ducks. DNA has been extracted from whole blood which was frozen immediately, Tris/NaCI/EDTA preserved tissue and heart and wing muscle which has been preserved in 70% ethanol and later stored in an utlrafreezer. Three multilocus probes, 33.6, 33....
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